Propidium Iodide Staining Principle

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The feature sheets represent the most advanced research with the most significant potential to have a significant impact in the field. Arrow papers are presented at the invitation or recommendation of each person by scientific editors and peer-reviewed before publication.

Propidium Iodide Staining Principle

Propidium Iodide Staining Principle

The content paper can be an original research article, a multi-novel study that often incorporates multiple approaches or methods, or a comprehensive review article with a brief and detailed review of recent developments in the field that systematically reviews the most exciting scientific advances. literature. This type of paper provides an overview of future research guidelines or potential applications.

Annexin Pi Assay For Apoptosis

Editorial selection The articles are based on the recommendations of scientific editors from journals around the world. The editors selected a small number of recently published articles in a journal that they thought would be of greater interest to the authors, or that would be more relevant to the field. The aim is to make a summary of the most interesting works published in various research fields in the journal.

Muhammad Din Ibrahim 1, Saie Brindha Kntayya 1, Nooraini Mohd Ain 1, Renato Iori 2, Costas Ioannides 3 and Ahmad Faizal Abdull Razis 4, 5, 6, *

CREA Agricultural Advisory and Analysis of the Agricultural Economy, Center for Agricultural Agriculture and the Environment (CREA-AA), Via di Corticella 133, 40128 Bologna, Italy

Food Safety and Food Resources, Tropical Agriculture and Food Safety Center, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

Inverted Microscopy And Acridin Orange/propidium Iodide Staining Of…

Glucorafasatin (GRH), the most abundant glucosinolate in plants of the Brassicaceae family, is hydrolyzed to raphasatin, which is responsible for the chemopreventive activity of myrosin in cancer; however, the basic mechanisms of action have not been investigated, especially in human cells. The aim of this study was to determine the cytotoxicity of raphasatin and to evaluate its potential for apoptosis and to regulate adenocarcinoma cell cycle adherence to MCF-7 cells. Cytotoxicity was established after insertion of cells containing glucorafasatin or raphasatin (0–100 µM) at 24, 48, and 72 hours. GRH showed no cytotoxicity as indicated by the 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) assay. When myrosinase was added to the incubation system to convert GRH to raphasatin, cytotoxicity was observed. Raphasatin cell exposure stimulates apoptosis, resulting in cell proliferation, membrane rupture, chromatin thickening, and nuclear rupture. Furthermore, using the Annexin V-FITC assay, raphasatin induces apoptosis, as evidenced by changes in cell distribution at different stages of apoptosis; In addition, raphasatin causes MCF-7 cells to bind to G

+ M category. As a result, raphasatin has shown potential for chemopreventive cancer against human breast adenocarcinoma (MCF-7) through the induction of apoptosis and the cessation of cell cycles.

Glucorafasatin; raphasatin; glucosinolates; isothiocyanates; linear cell adenocarcinoma; cytotoxicity; apoptosis; cell cycle glucorafasatin; raphasatin; glucosinolates; isothiocyanates; linear cell adenocarcinoma; cytotoxicity; apoptosis; cell cycle

Propidium Iodide Staining Principle

Cancer is a failure of the mechanisms that control the growth and division of cells, and remains the leading cause of death worldwide. Although chemo- and radiation treatments are commonly used in the treatment of cancer [1], these have serious side effects due to damage to the normal tissues surrounding the tumors [2]. In addition, abscesses may be resistant to this treatment [3]. Breast cancer is a very common disease among Malaysian women and worldwide, and 150-200 new cases are diagnosed each year at the Breast Clinic Hospital in Kuala Lumpur alone. It is estimated that one in 20 women will be diagnosed with breast cancer [4, 5]. Breast cancer accounts for 16.5% of all cancer cases registered in the Malaysian National Cancer Registry in 2006, and 34.4% of breast cancer cases recorded in 15-49 year olds aged 15-49 [6].

Hoechst 33342/propidium Iodide Staining Of Primary Rat Hepatocytes…

The importance of diet in the human etiology of human cancer has been well documented in recent years. Many phytochemicals, including ascorbic and phenolic acids, such as anthocyanins and flavonoids, have antioxidant activity and are considered to be important contributors to beneficial effects associated with the use of plant-rich plants. Broccoli (Brassica oleracea), radish (Raphanus sativus L.), kale and cabbage (B. oleracea L.) are examples of Brassica vegetables and glucosinolates rich in these antioxidants, which have recently attracted a great deal of attention. for years, due to the remarkable anti-carcinogenic activity of isothiocyanates, their main hydrolysis products [8, 9].

In numerous epidemiological studies, the use of cruciferous herbs is associated with a lower incidence of cancer in many areas [10, 11]. The chemopreventive properties of these herbs are due to glucosinolates, a class of sulfur containing glucosides in significant amounts [10]. Glucosinolates (GL) are the second metabolites of Brassica herbs that provide health-promoting benefits because of their hydrolysis products, isothiocyanates, chemopreventive and antioxidant properties [12].

4-Methylsulfanyl-3-butenyl glucosinolate, also known as GRH, is a glucosinolate, the main source of which is Raphanus sativus (Kaiware Daikon), a white radish widely used in Japan and widespread in Europe and North America [13], 14]. . Raphanus sativus L. radishes have aroused interest in the scientific community due to their phytochemical content in promoting health. Of particular interest is the isothiocyanate-rafasatin (Figure 1), which is released by hydrolysis, chewing, mowing, or other mechanical disturbances of fresh (uncooked) plant tissue [15]. In addition to the different taste of radish, isothiocyanates have indirect antioxidant effects that have chemopreventive activity and potentially therapeutic activity [16].

The ability to perform apoptosis is an important property of anti-cancer drugs. Various methods have been developed to test the ability of chemical agents to induce apoptosis, including morphological analysis, biochemical testing, ELISA, movement cytometry analysis, etc., in which morphological analysis appears to be more reliable [17]. Previous studies have shown that isothiocyanates show their chemopreventive activity, at least in part by properly modulating the processes of apoptosis and cell reproduction [18, 19, 20, 21], this study is designed to establish HRM capacity and its degradation. raphasatin production, apoptosis, and cell cycle arrest in adenocarcinoma cells (MCF-7).

Flow Cytometry Protocols

In this study, the function of MCF-7 cells was investigated after exposure to GRH and raphasatin. In the study areas (1–100 µM), HRM had no effect on MCF-7 cells (Table 1). In contrast, due to its degradation product, isothiocyanate-rafasatin was cytotoxic after incubation for 24, 48, and 72 hours. Paclitaxel (0-25 nM) acted as a good control and had a high cytotoxic effect on cells.

2.2. Morphological analysis of apoptosis using the deoxynucleotidyl transferase terminal dUTP Nick End Labeling (TUNEL) assay using orange acridine / propidium iodide (AO / PI) staining and 4 ′, 6-diamidino-2-phenylindole (DAPI) staining.

In morphological studies, TUNEL assays, AO / PI staining, and DAPI staining trials were used to test for morphological changes in MCF-7 cells under the influence of raphasatin at different times, 24, 48, and 72 h. The untreated cells (Figure 2A) in the TUNEL study were healthy, rotated, and invisible to TUNELs. After 24-hour treatment (Figure 2B), cells began to darken to indicate that other cells had developed apoptosis. After 48 hours of treatment (Figure 2C), the number of dark brown cells increased; about 50% of human cells have had apoptosis, which has resulted in the breakdown of nuclear DNA. Finally, cells treated at 72 h showed a high number of apoptotic cells, covering about 70% of human cells (Figure 2D).

Propidium Iodide Staining Principle

In AO / PI staining, untreated cells (Figure 3A) showed no morphological changes, and only light cells with a green, circular, and visible color showed no signs of apoptosis. However, apoptosis was clearly seen in the 24-hour treatment (Figure 3B) with raphasatin, where the first apoptosis with broken nucleus and chromatin condensation characteristics was observed, and round cells also began to contract. After 48 hours of treatment (Figure 3C), apoptosis was clear and membrane blebbing and chromatin condensation were observed. Bibles about the same cell indicate that apoptosis developed. In addition, some necrotic cells were clearly identified, along with red blood cells. Cells exposed to raphasatin for 72 hours (3D image) showed more severe apoptosis, including membrane blebbing, late apoptosis, secondary necrosis, and apoptotic cell formation. Furthermore, in the final stages of apoptosis, changes similar to the presence of red-orange color were observed as a result of binding to denatured AO DNA after 72 hours of treatment of the cells.

Fluorescein Diacetate (fda) And Propidium Iodide (pi) Can Be Used To…

As shown in Figure 4, treatment of DAPI contamination with MCF-7 cells with raphasatin at different times (24, 48, and 72 h) resulted in morphological symptoms similar to apoptotic cells, including nuclear dissociation, including the formation of micronuclei. , and chromatin condensation compared to control cells that maintain a spherical shape. In contrast, treated cells showed morphological symptoms

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